Recombinant mussel coating protein fused with cell adhesion recognition motif enhanced cell proliferation
SCIE
SCOPUS
KCI
- Title
- Recombinant mussel coating protein fused with cell adhesion recognition motif enhanced cell proliferation
- Authors
- Yoo, HY; Song, YH; Seo, JH; Cha, HJ; HWANG, DONG SOO
- Date Issued
- 2015-04
- Publisher
- The Korean Society for Biotechnology and Bioengineering
- Abstract
- Endurable coating on biomedical substrates is one of the most important issues in biomedical engineering field. Recently, a robust yet reversible coating has found in the cuticle of mussel byssus and the mimicking of the mussel cuticle coating has been considered as a strategy to build up endurable coating on biomedical materials. To date, the only known protein in the cuticle is mussel foot protein-1 (fp-1). To form endurable and bioactive coating on the biomedical surface, the fusion protein of fp-1 and GRGDSP peptide (fp-1-RGD) was genetically designed and produced in E. coli. The fusion protein of fp-1-RGD was successfully expressed as a form of inclusion body and was simply purified by diluted acetic acid extraction with high purity (similar to 95%). Fp-1-RGD was coated on the tissue culture polystyrene (TCPS) and showed better preosteoblast cell proliferation than that of TCPS. Therefore, the marriage of fp-1 and bioactive peptide can be a good strategy to form bioactive and endurable coating in biomedical field.
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/35521
- DOI
- 10.1007/S12257-014-0621-4
- ISSN
- 1226-8372
- Article Type
- Article
- Citation
- BIOTECHNOLOGY AND BIOPROCESS ENGINEERING, vol. 20, no. 2, page. 211 - 217, 2015-04
- Files in This Item:
- There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.