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Cited 12 time in webofscience Cited 9 time in scopus
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Roles of endothelial A-type lamins in migration of T cells on and under endothelial layers SCIE SCOPUS

Title
Roles of endothelial A-type lamins in migration of T cells on and under endothelial layers
Authors
Kwang Hoon SongJaehyun LeeHyoungJun ParkHye Mi KimJeehun ParkKeon Woo KwonDoh, J
Date Issued
2016-03-21
Publisher
NATURE PUBLISHING GROUP
Abstract
Stiff nuclei in cell-dense microenvironments may serve as distinct biomechanical cues for cell migration, but such a possibility has not been tested experimentally. As a first step addressing this question, we altered nuclear stiffness of endothelial cells (ECs) by reducing the expression of A-type lamins using siRNA, and investigated the migration of T cells on and under EC layers. While most T cells crawling on control EC layers avoided crossing over EC nuclei, a significantly higher fraction of T cells on EC layers with reduced expression of A-type lamins crossed over EC nuclei. This result suggests that stiff EC nuclei underlying T cells may serve as "duro-repulsive" cues to direct T cell migration toward less stiff EC cytoplasm. During subendothelial migration under EC layers with reduced expression of A-type lamins, T cells made prolonged contact and substantially deformed EC nuclei, resulting in reduced speed and directional persistence. This result suggests that EC nuclear stiffness promotes fast and directionally persistent subendothelial migration of T cells by allowing minimum interaction between T cells and EC nuclei.
Keywords
NUCLEAR MECHANICS; TRANSCELLULAR DIAPEDESIS; LYMPHOCYTE MIGRATION; LEUKOCYTE MIGRATION; FOCAL ADHESIONS; CANCER INVASION; VENULAR WALLS; IN-VIVO; INTERFERENCE; DISEASE
URI
https://oasis.postech.ac.kr/handle/2014.oak/29992
DOI
10.1038/SREP23412
ISSN
2045-2322
Article Type
Article
Citation
Scientific Reports, vol. 6, no. 23412, page. 1 - 13, 2016-03-21
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도준상DOH, JUN SANG
Dept of Mechanical Enginrg
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