TAGLN2 regulates T cell activation by stabilizing the actin cytoskeleton at the immunological synapse
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SCOPUS
- Title
- TAGLN2 regulates T cell activation by stabilizing the actin cytoskeleton at the immunological synapse
- Authors
- Na, BR; Kim, HR; Piragyte, I; Oh, HM; Kwon, MS; Akber, U; Lee, HS; Park, DS; Song, WK; Park, ZY; Im, SH; Rho, MC; Hyun, YM; Kim, M; Jun, CD
- Date Issued
- 2015-04-13
- Publisher
- ROCKEFELLER UNIV PRESS
- Abstract
- The formation of an immunological synapse (IS) requires tight regulation of actin dynamics by many actin polymerizing/depolymerizing proteins. However, the significance of actin stabilization at the IS remains largely unknown. In this paper, we identify a novel function of TAGLN2-an actin-binding protein predominantly expressed in T cells-in stabilizing cortical F-actin, thereby maintaining F-actin contents at the IS and acquiring LFA-1 (leukocyte function-associated antigen-1) activation after T cell receptor stimulation. TAGLN2 blocks actin depolymerization and competes with cofilin both in vitro and in vivo. Knockout of TAGLN2 (TAGLN2(-/-)) reduced F-actin content and destabilized F-actin ring formation, resulting in decreased cell adhesion and spreading. TAGLN2(-/-) T cells displayed weakened cytokine production and cytotoxic effector function. These findings reveal a novel function of TAGLN2 in enhancing T cell responses by controlling actin stability at the IS.
- Keywords
- ARP2/3 COMPLEX; F-ACTIN; RETROGRADE FLOW; PHORBOL ESTER; ALPHA-ACTININ; IN-VIVO; PROTEIN; COFILIN; PHOSPHORYLATION; ASSOCIATION
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/29897
- DOI
- 10.1083/JCB.201407130
- ISSN
- 0021-9525
- Article Type
- Article
- Citation
- JOURNAL OF CELL BIOLOGY, vol. 209, no. 1, page. 143 - 162, 2015-04-13
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