Colorimetric detection of penicillin antibiotic residues in pork using hybrid magnetic nanoparticles and penicillin class-selective, antibody-functionalized platinum nanoparticles
SCIE
SCOPUS
- Title
- Colorimetric detection of penicillin antibiotic residues in pork using hybrid magnetic nanoparticles and penicillin class-selective, antibody-functionalized platinum nanoparticles
- Authors
- Kwon, D; Lee, W; Kim, W; Yoo, H; Shin, HC; Jeon, S
- Date Issued
- 2015-01
- Publisher
- ROYAL SOC CHEMISTRY
- Abstract
- Here we describe the development of a rapid and facile method for the colorimetric detection of penicillin antibiotics, including penicillin G, penicillin V, nafcillin, oxacillin, cloxacillin and dicloxacillin, using hybrid magnetic nanoparticle clusters (HMNCs) and penicillin class-selective, antibody-functionalized platinum nanoparticles (Ab-Pt). Amine-functionalized HMNCs were used to chemically capture antibiotics from sample solution through the formation of amide bonds between amine groups of HMNCs and carboxyl groups of the antibiotics. Ab-Pt was then allowed to bind to antibiotic-HMNC complexes, after which the resulting Pt-antibiotic-HMNCs were added to a 3,3',5,5'-tetramethylbenzidine (TMB) solution. The color change caused by platinum nanoparticle-mediated oxidation of TMB enabled antibiotics to be identified with the naked eye and quantified by measuring light absorption. The use of the penicillin class-selective antibody allowed the simultaneous detection of penicillin class antibiotics, thereby reducing the cost, time, and effort associated with prescreening antibiotic residues in sample specimens.
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/29878
- DOI
- 10.1039/C5AY01590A
- ISSN
- 1759-9660
- Article Type
- Article
- Citation
- ANALYTICAL METHODS, vol. 7, no. 18, page. 7639 - 7645, 2015-01
- Files in This Item:
- There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.