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Parallel analysis of antimicrobial activities in microbial community by SSCP based on CE SCIE SCOPUS

Title
Parallel analysis of antimicrobial activities in microbial community by SSCP based on CE
Authors
Chung, JHPark, YSKim, JShin, GWNam, MHOh, MKKim, CWJung, GYPark, JH
Date Issued
2007-07
Publisher
WILEY-V C H VERLAG GMBH
Abstract
Conventional antimicrobial activity analyses such as the broth dilution method and disk diffusion test are considerably demanding processes for new antimicrobial agent discovery and sensitive diagnosis of infectious diseases. Here, we developed a new antimicrobial activity analysis system using CE-based SSCP (CE-SSCP) combined with 16S rRNA gene-specific PCR (PCR/CE-SSCP). Using this method, the population change in the microbial community in response to specific antimicrobial agents could be quantified with a high sensitivity and accuracy from a small sample amount. Using a mixture of microorganisms comprising Escherichia coli, Corynebacterium glutamicum, Acinetobacter calcoaceticus, and Staphylococcus aureus as a model system, the linear correlation between the genomic DNA concentrations and peak areas in 16S rRNA gene-specific PCR/CE-SSCP was determined; consequently, quantification of cell concentrations could be demonstrated using this method. Compared to the minimum inhibitory concentration (MIC) values from the conventional broth dilution method, this new system provided almost the same MIC values for popular antimicrobial agents such as kanamycin, spectinomycin, and streptomycin. The results demonstrated that the newly developed method can be a substitute for the conventional antimicrobial analysis method and highlighted its high potential in the areas of new antimicrobial agent discovery and clinical diagnosis.
Keywords
antimicrobial activity analysis; antimicrobial agent; CE; microbial community; 16S rRNA gene; SSCP; STRAND-CONFORMATION POLYMORPHISM; COMBINATORIAL CHEMISTRY; KINASE INHIBITORS; DRUG DISCOVERY; BACTERIAL; DISEASE; COMPLEX; ELECTROPHORESIS; CHALLENGES; PEPTIDES
URI
https://oasis.postech.ac.kr/handle/2014.oak/29465
DOI
10.1002/elps.200600570
ISSN
0173-0835
Article Type
Article
Citation
ELECTROPHORESIS, vol. 28, no. 14, page. 2416 - 2423, 2007-07
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정규열JUNG, GYOO YEOL
Dept. of Chemical Enginrg
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