X-ray imaging of various biological samples using a phase-contrast hard X-ray microscope
SCIE
SCOPUS
- Title
- X-ray imaging of various biological samples using a phase-contrast hard X-ray microscope
- Authors
- Kim, GB; Yoon, YJ; Shin, TJ; Youn, HS; Gho, YS; Lee, SJ
- Date Issued
- 2008-09
- Publisher
- WILEY-LISS
- Abstract
- In this study, we visualized the internal structures of various bio-samples and found the optimum conditions of test samples for the 7 keV hard X-ray microscope of the Pohang light source. From the captured X-ray images, we could observe the intercellular and intracellular structures of dehydrated human cells and mouse tumor tissues without using any staining materials in a spatial resolution better than 100 nm. The metastasized lung tissue, which was several tens of micrometers in thickness, was found to be very well suited to this hard X-ray microscope system, because it is nearly impossible to observe such a nontransparent and thick sample with a high spatial resolution better than 100 nm using any microscopes such as a soft X-ray microscope, an optical microscope, or an electron microscope.
- Keywords
- hard X-ray microscopy; phase-contrast; dehydrated cells; metastasized lung tissue; OBSERVING TRANSPARENT SPECIMENS
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/29369
- DOI
- 10.1002/JEMT.20601
- ISSN
- 1059-910X
- Article Type
- Article
- Citation
- MICROSCOPY RESEARCH AND TECHNIQUE, vol. 71, no. 9, page. 639 - 643, 2008-09
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