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Cited 11 time in webofscience Cited 11 time in scopus
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Quantitative oligonucleotide microarray data analysis with an artificial standard probe strategy SCIE SCOPUS

Title
Quantitative oligonucleotide microarray data analysis with an artificial standard probe strategy
Authors
Hwang, BHCha, HJ
Date Issued
2008-06-15
Publisher
ELSEVIER ADVANCED TECHNOLOGY
Abstract
Quantitative data analysis is an important element in several applications of DNA microarray, including mRNA expression profiling and estimation of infectious doses for pathogens. Here, we introduce an artificial standard probe strategy for quantitative pathogen detection using an oligonucleotide chip as a model system. The standard capture probe sequence was artificially designed to prevent non-specific hybridization with bacterial targets. Based on the fluorescence intensities of artificial standard spots, the raw fluorescence intensity data for specific spots could be corrected to generate linear correlations with target concentrations. Therefore, our novel artificial standard probe may be effectively applied for the correction of chip-to-chip variations and quantitative data analysis of a one-color labeled DNA microarray system. (c) 2008 Elsevier B.V. All rights reserved.
Keywords
DNA chip; oligonucleotide microarray; quantitative data analysis; artificial standard probe; DNA MICROARRAY; CDNA MICROARRAYS; MICROCHIPS; GENES; PCR
URI
https://oasis.postech.ac.kr/handle/2014.oak/29348
DOI
10.1016/j.bios.2008.01.024
ISSN
0956-5663
Article Type
Article
Citation
BIOSENSORS & BIOELECTRONICS, vol. 23, no. 11, page. 1738 - 1744, 2008-06-15
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차형준CHA, HYUNG JOON
Dept. of Chemical Enginrg
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