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DYNAMIC REGULATION OF NUCLEOSOME POSITIONING IN THE HUMAN GENOME SCIE SCOPUS

Title
DYNAMIC REGULATION OF NUCLEOSOME POSITIONING IN THE HUMAN GENOME
Authors
Schones, DECui, KRCuddapah, SRoh, TYBarski, AWang, ZBWei, GZhao, KJ
Date Issued
2008-03-07
Publisher
CELL PRESS
Abstract
The positioning of nucleosomes with respect to DNA plays an important role in regulating transcription. However, nucleosome mapping has been performed for only limited genomic regions in humans. We have generated genome-wide maps of nucleosome positions in both resting and activated human CD4(+) T cells by direct sequencing of nucleosome ends using the Solexa high-throughput sequencing technique. We find that nucleosome phasing relative to the transcription start sites is directly correlated to RNA polymerase II (Pol II) binding. Furthermore, the first nucleosome downstream of a start site exhibits differential positioning in active and silent genes. TCR signaling induces extensive nucleosome reorganization in promoters and enhancers to allow transcriptional activation or repression. Our results suggest that H2A.Z-containing and modified nucleosomes are preferentially lost from the -1 nucleosome position. Our data provide a comprehensive view of the nucleosome landscape and its dynamic regulation in the human genome.
Keywords
EMBRYONIC STEM-CELLS; HISTONE H2A VARIANT; RNA-POLYMERASE; DEVELOPMENTAL REGULATORS; SACCHAROMYCES-CEREVISIAE; STRUCTURAL-ANALYSIS; CHROMATIN-STRUCTURE; SWI/SNF COMPLEX; IN-VIVO; WIDE
URI
https://oasis.postech.ac.kr/handle/2014.oak/28992
DOI
10.1016/J.CELL.2008.
ISSN
0092-8674
Article Type
Article
Citation
CELL, vol. 132, no. 5, page. 887 - 898, 2008-03-07
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노태영ROH, TAE YOUNG
Dept of Life Sciences
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