PROTEOME ANALYSIS OF OUTER MEMBRANE VESICLES FROM A CLINICAL ACINETOBACTER BAUMANNII ISOLATE
SCIE
SCOPUS
- Title
- PROTEOME ANALYSIS OF OUTER MEMBRANE VESICLES FROM A CLINICAL ACINETOBACTER BAUMANNII ISOLATE
- Authors
- Kwon, SO; Gho, YS; Lee, JC; Kim, SI
- Date Issued
- 2009-08
- Publisher
- Blackwell Publishing Ltd
- Abstract
- The secretion of outer membrane vesicles (OMVs) is one of the major mechanisms by which Gram-negative bacteria deliver effector molecules to host cells. Acinetobacter baumannii is an important opportunistic pathogen in hospital-acquired infections, but the secretion system for effector molecules to induce host cell damage has not been characterized. In the present study, we investigated the secretion of OMVs from a clinical A. baumannii isolate and analyzed the comprehensive proteome of A. baumannii-derived OMVs. Acinetobacter baumannii secreted OMVs into the extracellular milieu during in vitro growth. Using 1-DE and LC-MS/MS protein identification and assignment analysis, 132 different proteins associated with OMVs were identified. These proteins were derived from outer membranes (n=26), periplasmic space (n=6), inner membranes (n=8), cytoplasm (n=43), and unknown localization or multiple localization sites (n=49) according to the cell location prediction programs. Among the proteins associated with OMVs, a potent cytotoxic molecule, outer membrane protein A, was highly enriched and several putative virulence-associated proteins were also identified. These results suggest that OMVs from A. baumannii are an important vehicle designed to deliver effector molecules to host cells.
- Keywords
- proteome; outer membrane protein A; bacterial secretion system; virulence factor; EPITHELIAL-CELLS; PSEUDOMONAS-AERUGINOSA; ACTIVATION; BACTERIUM; GENTAMICIN; PROTEINS; GROWTH; DU202
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/28768
- DOI
- 10.1111/j.1574-6968.2009.01669.x
- ISSN
- 0378-1097
- Article Type
- Article
- Citation
- FEMS MICROBIOLOGY LETTERS, vol. 297, no. 2, page. 150 - 156, 2009-08
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