ALTERNATING-LASER EXCITATION OF SINGLE MOLECULES
SCIE
SCOPUS
- Title
- ALTERNATING-LASER EXCITATION OF SINGLE MOLECULES
- Authors
- Kapanidis, AN; Laurence, TA; Lee, NK; Margeat, E; Kong, XX; Weiss, S
- Date Issued
- 2005-07
- Publisher
- AMER CHEMICAL SOC
- Abstract
- Single-molecule fluorescence spectroscopy addresses biological mechanisms and enables ultrasensitive diagnostics. We describe a new family of single-molecule fluorescence methods that uses alternating-laser excitation (ALEX) of diffusing or immobilized biomolecules to study their structure, interactions, and dynamics. This is accomplished using ratios that report on the distance between and the smichiometry of fluorophores attached to the molecules of interest. The principle of alternation is compatible with several time scales, allowing monitoring of fast dynamics or simultaneous monitoring of a large number of individual molecules.
- Keywords
- RESONANCE ENERGY-TRANSFER; FLUORESCENCE SPECTROSCOPY; CONFORMATIONAL DYNAMICS; RNA-POLYMERASE; DNA-MOLECULES; BIOMOLECULES; MICROSCOPY; COMPLEX; CELLS
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/28423
- DOI
- 10.1021/AR0401348
- ISSN
- 0001-4842
- Article Type
- Article
- Citation
- ACCOUNTS OF CHEMICAL RESEARCH, vol. 38, no. 7, page. 523 - 533, 2005-07
- Files in This Item:
- There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.