SINGLE-MOLECULE APPROACH TO MOLECULAR BIOLOGY IN LIVING BACTERIAL CELLS
SCIE
SCOPUS
- Title
- SINGLE-MOLECULE APPROACH TO MOLECULAR BIOLOGY IN LIVING BACTERIAL CELLS
- Authors
- Xie, XS; Choi, PJ; Li, GW; Lee, NK; Lia, G
- Date Issued
- 2008-06
- Publisher
- ANNUAL REVIEWS
- Abstract
- Recent developments on fluorescent proteins and microscopy techniques have allowed the probing of single molecules in a living bacteria] cell with high specificity, millisecond time resolution, and nanometer spatial precision. Recording movies and analyzing dynamics of individual macromolecules have brought new insights into the mechanisms of many processes in molecular biology, such is DNA-protein interactions, gene regulation, transcription, translation, and replication, among others. Here we review the key methods of single-molecule detection and highlight numerous examples to illustrate how these experiments are contributing to the quantitative understanding of the fundamental processes in a living cell.
- Keywords
- living cells; stochastic gene expression; DNA-protein interactions; transcription; translation; replication; FLUORESCENCE CORRELATION SPECTROSCOPY; FACILITATED TARGET LOCATION; COLI CHROMOSOME ARMS; ESCHERICHIA-COLI; GENE-EXPRESSION; DNA-POLYMERASE; LAC REPRESSOR; STRAND SYNTHESIS; RECA PROTEIN; TRANSCRIPTION
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/28397
- DOI
- 10.1146/ANNUREV.BIOP
- ISSN
- 1936-122X
- Article Type
- Article
- Citation
- ANNUAL REVIEW OF BIOPHYSICS AND BIOMOLECULAR STRUCTURE, vol. 37, page. 417 - 444, 2008-06
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