Presynaptic activation of silent synapses and growth of new synapses contribute to intermediate and long-term facilitation in Aplysia
SCIE
SCOPUS
- Title
- Presynaptic activation of silent synapses and growth of new synapses contribute to intermediate and long-term facilitation in Aplysia
- Authors
- Kim, JH; Udo, H; Li, HL; Youn, TY; Chen, M; K; el, ER; Bailey, CH
- Date Issued
- 2003-09-25
- Publisher
- CELL PRESS
- Abstract
- The time course and functional significance of the structural changes associated with long-term facilitation of Aplysia sensory to motor neuron synaptic connections in culture were examined by time-lapse confocal imaging of individual sensory neuron varicosities labeled with three different fluorescent markers: the whole-cell marker Alexa-594 and two presynaptic marker proteins-synaptophysin-eGFP to monitor changes in synaptic vesicle distribution and synapto-PHluorin to monitor active transmitter release sites. Repeated pulses of serotonin induce two temporally, morphologically, and molecularly distinct presynaptic changes: (1) a rapid activation of silent presynaptic terminals by filling of preexisting empty varicosities with synaptic vesicles, which parallels intermediate-term facilitation, is completed within 3-6 hr and requires translation but not transcription and (2) a slower generation of new functional varicosities which occurs between 12-18 hr and requires transcription and translation. Enrichment of empty varicosities with synaptophysin accounts for 32% of the newly activated synapses at 24 hr, whereas newly formed varicosities account for 68%.
- Keywords
- LOCAL PROTEIN-SYNTHESIS; SYNAPTIC PLASTICITY; STRUCTURAL-CHANGES; SENSORY NEURONS; MEMORY STORAGE; HETEROSYNAPTIC FACILITATION; MORPHOLOGICAL-CHANGES; MOTOR SYNAPSES; CELL-CULTURE; TIME-COURSE
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/28381
- DOI
- 10.1016/S0896-6273(03)00595-6
- ISSN
- 0896-6273
- Article Type
- Article
- Citation
- NEURON, vol. 40, no. 1, page. 151 - 165, 2003-09-25
- Files in This Item:
- There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.