Cationic derivatives of biocompatible hyaluronic acids for delivery of siRNA and antisense oligonucleotides
- Title
- Cationic derivatives of biocompatible hyaluronic acids for delivery of siRNA and antisense oligonucleotides
- Authors
- HAN, SE; KANG, H; SHIM, GY; KIM, SJ; CHOI, HG; KIM, J; HAHN, SK; OH, YK; null
- Date Issued
- 2009-02
- Publisher
- TAYLOR & FRANCIS LTD
- Abstract
- In this study, we tested the use of cationic polymer derivatives of biocompatible hyaluronic acid (HA) as a delivery system of siRNA and antisense oligonucleotides. HA was modified with cationic polymer polyethylenimine (PEI). When compared with PEI alone, cationic PEI derivatives of HA (HA-PEI) provided increased cellular delivery of Small interfering RNA (siRNA) in B16F1, A549, HeLa, and Hep3B tumor cells. Indeed, more than 95% of the cells were positive for siRNA following its delivery with HA-PEI. A survivin-specific siRNA that was delivered using HA-PEI potently reduced the mRNA expression levels of the target gene in all of the cell lines. By contrast, survivin-specific siRNA delivered by PEI alone did not induce a significant reduction in mRNA levels. In green fluorescent protein (GFP)-expressing 293 T cells, a loss of GFP expression was evident in the cells that had been treated with GFP-specific siRNA and HA-PEI complex. The inhibition of target gene expression by antisense oligonucleotide 63139 was also enhanced after delivery with HA-PEI. Moreover, HA-PEI displayed lower cytotoxicity than PEI alone. These results suggest that HA-PEI could be further developed as biocompatible delivery systems of siRNA and antisense oligonucleotides for enhanced cellular uptake and inhibition of target gene expression.
- Keywords
- Hyaluronic acid; cationic polymer; siRNA delivery; antisense oligonucleotides; PLASMID DNA; CELLULAR UPTAKE; IN-VIVO; POLYETHYLENIMINE; CELLS; EXPRESSION; COMPLEXES; CANCER
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/28334
- DOI
- 10.1080/106118608024
- ISSN
- 1061-186X
- Article Type
- Article
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- There are no files associated with this item.
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