Homogeneous differentiation of hepatocyte-like cells from embryonic stem cells: applications for the treatment of liver failure
SCIE
SCOPUS
- Title
- Homogeneous differentiation of hepatocyte-like cells from embryonic stem cells: applications for the treatment of liver failure
- Authors
- Cho, CH; Parashurama, N; Park, EYH; Suganuma, K; Nahniias, Y; Park, J; Tilles, AW; Berthiaume, F; Yarmush, ML
- Date Issued
- 2008-03
- Publisher
- FEDERATION AMER SOC EXP BIOL
- Abstract
- One of the major hurdles of cellular therapies for the treatment of liver failure is the low availability of functional human hepatocytes. While embryonic stem (ES) cells represent a potential cell source for therapy, current methods for differentiation result in mixed cell populations or low yields of the cells of interest. Here we describe a rapid, direct differentiation method that yields a homogeneous population of endoderm-like cells with 95% purity. Mouse ES cells cultured on top of collagen-sandwiched hepatocytes differentiated and proliferated into a uniform and homogeneous cell population of endoderm-like cells. The endoderm-like cell population was positive for Foxa2, Sox17, and AFP and could be further differentiated into hepatocyte-like cells, demonstrating hepatic morphology, functionality, and gene and protein expression. Incorporating the hepatocyte-like cells into a bioartificial liver device to treat fulminant hepatic failure improved animal survival, thereby underscoring the therapeutic potential of these cells.-Cho, C. H., Parashurama, N., Park, E. Y. H., Sugamuna, K, Nahmias, Y., Park, J., Tilles, A. W., Berthiaume, F., Yarmush, M. L. Homogeneous differentiation of hepatocyte-like cells from embryonic stem cells: applications for the treatment of liver failure.
- Keywords
- coculture; endoderm; animal model; bioartificial liver device; IN-VITRO DIFFERENTIATION; DEFINITIVE ENDODERM; GROWTH-FACTOR; HEPATIC DIFFERENTIATION; SANDWICH CONFIGURATION; BIOARTIFICIAL LIVER; COLLAGEN SANDWICH; ENDOTHELIAL-CELLS; CONTROLLED-TRIAL; GENE-EXPRESSION
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/28198
- DOI
- 10.1096/FJ.06-7764COM
- ISSN
- 0892-6638
- Article Type
- Article
- Citation
- FASEB JOURNAL, vol. 22, no. 3, page. 898 - 909, 2008-03
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