pH-Dependent Structures of an i-Motif DNA in Solution
SCIE
SCOPUS
- Title
- pH-Dependent Structures of an i-Motif DNA in Solution
- Authors
- Jin, KS; Shin, SR; Ahn, B; Rho, Y; Kim, SJ; Ree, M
- Date Issued
- 2009-02-19
- Publisher
- AMER CHEMICAL SOC
- Abstract
- We have investigated for the first time the structure of i-motif DNA in solution at various pH conditions by using synchrotron small-angle X-ray scattering technique. To facilitate direct structural. comparison between solution structures of i-motif DNA at various pH values, we created atomic coordinates of i-motif DNA from a fully folded to unfolded atomic model. Under mild acidic conditions, the conformations for i-motif DNA appeared to be similar to that of the partially unfolded i-motif atomic model in overall shape, rather than the fully folded i-motif atomic model. Collectively, our observations indicate that i-motif DNA molecule is structurally dynamic over a wide pH range, adopting multiple conformations ranging from the folded i-motif structure to a random coil conformation. As the i-motif structure has been used as an important component in nanomachines, we can therefore believe that the structural evidence presented herein will promote the development of future DNA-based molecular-actuator devices.
- Keywords
- X-RAY-SCATTERING; ORGANOSILICATE DIELECTRIC FILMS; COPOLYMER THIN-FILMS; BIOLOGICAL MACROMOLECULES; DIBLOCK COPOLYMER; CRYSTAL-STRUCTURE; ENERGY-TRANSFER; EVOLUTION; MACHINES; POLYPEPTIDE
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/27556
- DOI
- 10.1021/JP808186Z
- ISSN
- 1520-6106
- Article Type
- Article
- Citation
- JOURNAL OF PHYSICAL CHEMISTRY B, vol. 113, no. 7, page. 1852 - 1856, 2009-02-19
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