Grease matrix as a versatile carrier of proteins for serial crystallography
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- Title
- Grease matrix as a versatile carrier of proteins for serial crystallography
- Authors
- Sugahara, M; Mizohata, E; Nango, E; Suzuki, M; Tanaka, T; Masudala, T; Tanaka, R; Shimamura, T; Tanaka, Y; Suno, C; Ihara, K; Pan, D; Kakinouchi, K; Sugiyama, S; Murata, M; Inoue, T; Tono, K; Song, C; Park, J; Kameshima, T; Hatsui, T; Joti, Y; Yabashi, M; Iwata, S
- Date Issued
- 2015-01
- Publisher
- NPG
- Abstract
- Serial femtosecond X-ray crystallography (SFX) has revolutionized atomic-resolution structural investigation by expanding applicability to micrometer-sized protein crystals, even at room temperature, and by enabling dynamics studies. However, reliable crystal-carrying media for SFX are lacking. Here we introduce a grease-matrix carrier for protein microcrystals and obtain the structures of lysozyme, glucose isomerase, thaumatin and fatty acid-binding protein type 3. under ambient conditions at a resolution of or finer than 2 angstrom.
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/26703
- DOI
- 10.1038/NMETH.3172
- ISSN
- 1548-7091
- Article Type
- Article
- Citation
- NATURE METHODS, vol. 12, no. 1, page. 61 - 63, 2015-01
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- There are no files associated with this item.
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