Map-based cloning of the ERECT PANICLE 3 gene in rice
SCIE
SCOPUS
- Title
- Map-based cloning of the ERECT PANICLE 3 gene in rice
- Authors
- Piao, R; Jiang, W; Ham, TH; Choi, MS; Qiao, Y; Chu, SH; Park, JH; Woo, MO; Jin, Z; An, G; Lee, J; Koh, HJ
- Date Issued
- 2009-11
- Publisher
- SPRINGER
- Abstract
- Panicle architecture in rice can have a strong influence on yield. Using N-methyl-N-nitrosourea mutagenesis, we isolated an erect panicle mutant, Hep, from Hwasunchalbyeo, a glutinous japonica rice cultivar. Genetic analysis revealed that the erect panicle phenotype was controlled by a single recessive mutation designated erect panicle 3 (ep3). Genetic mapping revealed that the ep3 mutation was located on the short arm of chromosome 2 in a 0.1 cM region delimited by the STS markers STS5803-5 and STS5803-7. The ep3 locus corresponded to 46.8 kb region and contained six candidate genes. Comparison of the DNA sequences of the candidate genes from wild-type and erect panicle plants revealed a single base-pair change in the second exon of LOC_Os02g15950, which is predicted to result in a nonsense mutation. LOC_Os02g15950 encodes a putative F-box protein containing 515 amino acids and is expressed throughout the plant during all growth stages. A line carrying a T-DNA insertion in LOC_ Os02g15950 was obtained and shown to have the same phenotype as the ep3 mutant, thus confirming the identification of LOC_Os02g15950 as the ERECT PANICLE 3 (EP3) gene. The ep3 mutation causes a significant increase in the number of small vascular bundles as well as the thickness of parenchyma in the peduncle, which results in the erect panicle phenotype.
- Keywords
- F-BOX PROTEINS; DISEASE-RESISTANCE; MERISTEM IDENTITY; CIRCADIAN CLOCK; ARABIDOPSIS; ABERRANT-PANICLE-ORGANIZATION-1; IDENTIFICATION; POPULATIONS; EXPRESSION; INTERACTS
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/26514
- DOI
- 10.1007/S00122-009-1151-X
- ISSN
- 0040-5752
- Article Type
- Article
- Citation
- THEORETICAL AND APPLIED GENETICS, vol. 119, no. 8, page. 1497 - 1506, 2009-11
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