Collapsin response mediator protein-2 regulates neurite formation by modulating tubulin GTPase activity
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SCOPUS
- Title
- Collapsin response mediator protein-2 regulates neurite formation by modulating tubulin GTPase activity
- Authors
- Chae, YC; Lee, S; Heo, K; Ha, SH; Jung, Y; Kim, JH; Ihara, Y; Suh, PG; Ryu, SH
- Date Issued
- 2009-12
- Publisher
- ELSEVIER SCIENCE INC
- Abstract
- Collapsin response mediator protein-2 (CRMP-2) plays a key role in axonal development by regulating microtubule dynamics. However, the molecular mechanisms underlying this function have not been clearly elucidated. In this study, we demonstrated that hCRMP-2, specifically amino acid residues 480-509, is essential for stimulating tubulin GTPase activity. We also found that the GTPase-activating protein (GAP) activity of hCRMP-2 was important for microtubule assembly and neurite formation in differentiated PC12 pheochromocytoma cell lines. Mutant hCRMP-2, lacking arginine residues responsible for GAP activity, inhibited microtubule assembly and neurite formation. Interestingly, we found that the N-terminal region (amino acids 150-299) of hCRMP-2 had an inhibitory role on GAP activity via a direct interaction with the C-terminal region (amino acids 480-509). Our results suggest that CRMP-2 as a tubulin direct binder may be a GAP of tubulin in neurite formation and that its CAP activity may be regulated by an intramolecular interaction with an N-terminal inhibitory region. (C) 2009 Elsevier Inc. All rights reserved.
- Keywords
- CRMP-2; Tubulin; GTPase activity; Regulation; Microtubule; MICROTUBULE POLYMERIZATION DYNAMICS; GROWTH CONE COLLAPSE; CRMP-2; PHOSPHORYLATION; GUIDANCE; NEURONS; UNC-33; OUTGROWTH; RAT
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/26281
- DOI
- 10.1016/J.CELLSIG.2009.07.017
- ISSN
- 0898-6568
- Article Type
- Article
- Citation
- CELLULAR SIGNALLING, vol. 21, no. 12, page. 1818 - 1826, 2009-12
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