A novel organophosphorus hydrolase-based biosensor using mesoporous carbons and carbon black for the detection of organophosphate nerve agents
SCIE
SCOPUS
- Title
- A novel organophosphorus hydrolase-based biosensor using mesoporous carbons and carbon black for the detection of organophosphate nerve agents
- Authors
- Lee, JH; Park, JY; Min, K; Cha, HJ; Choi, SS; Yoo, YJ
- Date Issued
- 2010-03-15
- Publisher
- ELSEVIER ADVANCED TECHNOLOGY
- Abstract
- To detect organophosphate chemicals, which are used both as pesticides and as nerve agents, a novel biosensor based on organo phosphorus hydrolase was developed. By using mesoporous carbon (MC) and carbon black (CB) as an anodic layer, the sensitivity of the sensor to p-nitrophenol (PNP), which is the product of the organophosphorus hydrolase reaction, was greatly improved. The MC/CB/glass carbon (GC) layer exhibited an enhanced amperometric response relative to a carbon nanotube (CNT)-modified electrode because it promoted electron transfer of enzymatically generated phenolic compounds (p-nitrophenol). The well-ordered nanopores, many edge-plane-like defective sites (EDSs), and high surface area of the MC resulted in increased sensitivity, and allowed for nanomolar-range detection of the analyte paraoxon. Thus, MCs are suitable for use in real-time biosensors. Under the optimized experimental conditions, the biosensor had a detection limit of 0.12 mu M (36 ppb) and a sensitivity of 198 nA/mu M for paraoxon. (C) 2009 Elsevier B.V. All rights reserved.
- Keywords
- Organaphosphorous hydrolase; Organophosphate pesticides; Carbon nanotube; Biosensor; Mesoporous carbon; ELECTRODE MATERIAL; NANOTUBES; GRAPHITE; PESTICIDES; SITES; ENDS
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/26248
- DOI
- 10.1016/J.BIOS.2009.10.013
- ISSN
- 0956-5663
- Article Type
- Article
- Citation
- BIOSENSORS & BIOELECTRONICS, vol. 25, no. 7, page. 1566 - 1570, 2010-03-15
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