Susceptibility to Simian Immunodeficiency Virus Ex Vivo Predicts Outcome of a Prime-Boost Vaccine After SIVmac239 Challenge
SCIE
SCOPUS
- Title
- Susceptibility to Simian Immunodeficiency Virus Ex Vivo Predicts Outcome of a Prime-Boost Vaccine After SIVmac239 Challenge
- Authors
- Ochieng, W; Sauermann, U; Schulte, R; Suh, YS; Kim, KS; Sung, YC; Hunsmann, G; Stahl-Hennig, C; Sopper, S
- Date Issued
- 2009-10-01
- Publisher
- LIPPINCOTT WILLIAMS & WILKINS
- Abstract
- Background: Efficacy assessment of AIDS vaccines relies both on preclinically challenging immunized monkeys with simian immunodeficiency virus (SIV) or monitoring infection rates in large human trials. Although conventional parameters of vaccine-induced immune responses do not completely predict outcome, existing methods for testing cellular immunity are sophisticated and difficult to establish in resource-limited settings. Methods: We have used virus replication kinetics (VVR) on ConA-stimulated peripheral blood mononuclear cells from rhesus monkeys immunized with DNA replication-defective adenovirus vector expressing various SIV genes, as an ex vivo model, to mimic the effects of different immune effector functions on viral infection. Results: VVR was attenuated by the immunization and correlated 2 weeks after first boost, with the number of interferon gamma-secreting cells and T-cell noncytotoxic antiviral responses. Importantly, VVR on the day of challenge but not interferon gamma responses correlated with viremia and with memory CD4+ T-cell measurements after SIVmac239 challenge. Similarly, T-cell noncytotoxic antiviral responses on the day of challenge correlated directly with memory CD4(+) T cell and inversely with plasma viremia after challenge. Conclusions: VVR thus served as a better predictor of protective capacity of the vaccine regimen in these monkeys. We suggest that VVR be considered in the evaluation of candidate AIDS vaccines in humans.
- Keywords
- correlate of protection; HIV; in vitro replication; immune response; immunization; ANTI-HIV RESPONSE; T-CELL RESPONSES; RHESUS MACAQUES; IN-VITRO; DISEASE PROGRESSION; IMMUNE-RESPONSES; REPLICATION; INFECTION; DNA; MONKEYS
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/26089
- DOI
- 10.1097/QAI.0b013e3181b22f4a
- ISSN
- 1525-4135
- Article Type
- Article
- Citation
- JAIDS-JOURNAL OF ACQUIRED IMMUNE DEFICIENCY SYNDROMES, vol. 52, no. 2, page. 162 - 169, 2009-10-01
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