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Homomeric interaction of AtVSR1 is essential for its function as a vacuolar sorting receptor. SCIE SCOPUS

Title
Homomeric interaction of AtVSR1 is essential for its function as a vacuolar sorting receptor.
Authors
Kim, HKang, HJang, MChang, JMiao, YSJiang, LWHwang, I
Date Issued
2010-09
Publisher
AMER SOC PLANT BIOLOGISTS
Abstract
Vacuolar sorting receptors, BP80/VSRs, play a critical role in vacuolar trafficking of soluble proteins in plant cells. However, the mechanism of action of BP80 is not well understood. Here, we investigate the action mechanism of AtVSR1, a member of BP80 proteins in Arabidopsis (Arabidopsis thaliana), in vacuolar trafficking. AtVSR1 exists as multiple forms, including a high molecular mass homomeric complex in vivo. Both the transmembrane and carboxyl-terminal cytoplasmic domains of AtVSR1 are necessary for the homomeric interaction. The carboxyl-terminal cytoplasmic domain contains specific sequence information, whereas the transmembrane domain has a structural role in the homomeric interaction. In protoplasts, an AtVSR1 mutant, C2A, that contained alanine substitution of the region involved in the homomeric interaction, was defective in trafficking to the prevacuolar compartment and localized primarily to the trans-Golgi network. In addition, overexpression of C2A, but not wild-type AtVSR1, inhibited trafficking of soluble proteins to the vacuole and caused their secretion into the medium. Furthermore, C2A: hemagglutinin in transgenic plants interfered with the homomeric interaction of endogenous AtVSR1 and inhibited vacuolar trafficking of sporamin: green fluorescent protein. These data suggest that homomeric interaction of AtVSR1 is critical for its function as a vacuolar sorting receptor.
Keywords
TRANS-GOLGI NETWORK; MANNOSE 6-PHOSPHATE RECEPTOR; SOLUBLE CARGO PROTEINS; PREVACUOLAR COMPARTMENT; PLANT-CELLS; ENDOPLASMIC-RETICULUM; ARABIDOPSIS-THALIANA; TARGETING RECEPTOR; SECRETORY PATHWAY; CYTOPLASMIC TAIL
URI
https://oasis.postech.ac.kr/handle/2014.oak/25775
DOI
10.1104/PP.110.159814
ISSN
0032-0889
Article Type
Article
Citation
PLANT PHYSIOLOGY, vol. 154, no. 1, page. 134 - 148, 2010-09
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황인환HWANG, INHWAN
Dept of Life Sciences
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