Proteomic Analysis of Tumor Necrosis Factor-alpha-Induced Secretome of Human Adipose Tissue-Derived Mesenchymal Stem Cells
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SCOPUS
- Title
- Proteomic Analysis of Tumor Necrosis Factor-alpha-Induced Secretome of Human Adipose Tissue-Derived Mesenchymal Stem Cells
- Authors
- Lee, MJ; Kim, J; Kim, MY; Bae, YS; Ryu, SH; Lee, TG; Kim, JH
- Date Issued
- 2010-04
- Publisher
- AMER CHEMICAL SOC
- Abstract
- Human adipose tissue-derived mesenchymal stem cells (hASCs) are useful for regeneration of inflamed or injured tissues. To identify secreted hASC proteins during inflammation, hASCs were exposed to tumor necrosis factor-alpha (TNF-alpha) and conditioned media derived from hASCs were analyzed by liquid chromatography coupled with tandem mass spectrometry. We identified 187 individual proteins as secreted proteins (secretome) in hASC-conditioned media; 118 proteins were secreted at higher levels upon TNF-a treatment. The TNF-alpha-induced secretome included a variety of cytokines and chemokines such as interleukin-6 (IL-6), IL-8, chemokine (C-X-C motif) ligand 6, and monocyte chemotactic protein-1 (MCP-1). TNF-alpha also increased expression of various proteases including cathepsin L, matrix metalloproteases and protease inhibitors, and induced secretion of long pentraxin 3, a key inflammatory mediator implicated in innate immunity. TNF-alpha-conditioned media stimulated migration of human monocytes, which play a key role in inflammatory responses. This migration was abrogated by pretreatment with neutralizing anti-IL-6, anti-IL-8, and anti-MCP-1 antibodies, suggesting that IL-6, IL-8, and MCP-1 are involved in migration of monocytes. Taken together, these results suggest that TNF-alpha-induced secretome may play a pivotal role in inflammatory responses and that shotgun proteomic analysis will be useful for elucidation of the paracrine functions of mesenchymal stem cells.
- Keywords
- mesenchymal stem cells; secretome; TNF-alpha; monocytes; chemotaxis; LONG PENTRAXIN PTX3; HUMAN BONE-MARROW; STROMAL CELLS; CYSTEINE CATHEPSINS; MASS-SPECTROMETRY; LABEL-FREE; EXPRESSION; DISEASE; INTERLEUKIN-6; ACTIVATION
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/25690
- DOI
- 10.1021/PR900898N
- ISSN
- 1535-3893
- Article Type
- Article
- Citation
- JOURNAL OF PROTEOME RESEARCH, vol. 9, no. 4, page. 1754 - 1762, 2010-04
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