A membranous form of ICAM-1 on exosomes efficiently blocks leukocyte adhesion to activated endothelial cells
SCIE
SCOPUS
- Title
- A membranous form of ICAM-1 on exosomes efficiently blocks leukocyte adhesion to activated endothelial cells
- Authors
- Lee, HM; Choi, EJ; Kim, JH; Kim, TD; Kim, YK; Kang, C; Gho, YS
- Date Issued
- 2010-06-25
- Publisher
- ACADEMIC PRESS INC ELSEVIER SCIENCE
- Abstract
- While intercellular adhesion molecule-1 (ICAM-1) is a transmembrane protein, two types of extracellular ICAM-1 have been detected in cell culture supernatants as well as in the serum: a soluble form of ICAM-1 (sICAM-1) and a membranous form of ICAM-1 (mICAM-1) associated with exosomes. Previous observations have demonstrated that sICAM-1 cannot exert potent immune modulatory activity due to its low affinity for leukocyte function-associated antigen-1 (LFA-1) or membrane attack complex-1. In this report, we initially observed that human cancer cells shed mICAM-1(+)-exosomes but were devoid of vascular cell adhesion molecule-1 and E-selectin. We demonstrate that mICAM-1 on exosomes retained its topology similar to that of cell surface ICAM-1, and could bind to leukocytes. In addition, we show that exosomal mICAM-1 exhibits potent anti-leukocyte adhesion activity to tumor necrosis factor-a-activated endothelial cells compared to that of sICAM-1. Taken together with previous findings, our results indicate that mICAM-1 on exosomes exhibits potent immune modulatory activity. (C) 2010 Elsevier Inc. All rights reserved.
- Keywords
- ICAM-1; Exosomes; Microparticles; Communicasome; Inflammation; Endothelial cell; GENE-EXPRESSION; CANCER CELLS; MOLECULE-1; MICROVESICLES; VESICLES; MICROPARTICLES; DIMERIZATION; DISTINCT; SICAM-1
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/25593
- DOI
- 10.1016/J.BBRC.2010.05.094
- ISSN
- 0006-291X
- Article Type
- Article
- Citation
- BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, vol. 397, no. 2, page. 251 - 256, 2010-06-25
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- There are no files associated with this item.
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