Extracellular vesicles are key intercellular mediators in the development of immune dysfunction to allergens in the airways
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- Title
- Extracellular vesicles are key intercellular mediators in the development of immune dysfunction to allergens in the airways
- Authors
- Shin, TS; Kim, JH; Kim, YS; Jeon, SG; Zhu, Z; Gho, YS; Kim, YK
- Date Issued
- 2010-10
- Publisher
- WILEY-BLACKWELL PUBLISHING, INC
- Abstract
- P>Background: Previous evidence indicates that inhalation of lipopolysaccharide (LPS)-containing with allergens induced mixed Th1 and Th17 cell responses in the airways. Extracellular vesicles (EVs) are nanometer-sized spherical, lipid-bilayered structures and are recently in the public eye as an intercellular communicator in immune responses. Objective: To evaluate the role of EVs secreted by LPS inhalation in the development of airway immune dysfunction in response to allergens. Methods: Extracellular vesicles in bronchoalveolar lavage fluids of BALB/c mice were isolated and characterized 24 h after applications to the airway of 10 mu g of LPS for 3 days. To evaluate the role of LPS-induced EVs on the development of airway immune dysfunction, in vivo and in vitro experiments were performed using the isolated LPS-induced EVs. Results: The inhalation of LPS enhanced EVs release into the BAL fluid, when compared to the application of PBS. Airway sensitization with allergens and LPS-induced EVs resulted in a mixed Th1 and Th17 cell responses, although that with allergens and PBS-induced EVs induced immune tolerance. In addition, LPS-induced EVs enhanced the production of Th1- and Th17-polarizing cytokines (IL-12p70 and IL-6, respectively) by lung dendritic cells. Moreover, the immune responses induced by the LPS-induced EVs were blocked by denaturation of the EV-bearing proteins. Conclusion: These data suggest that EVs (especially, the protein components) secreted by LPS inhalation are a key intercellular communicator in the development of airway immune dysfunction to inhaled LPS-containing allergens.
- Keywords
- asthma; airway immune dysfunction; extracellular vesicles; lipopolysaccharide; DENDRITIC CELLS; EXPERIMENTAL ASTHMA; MEMBRANE-VESICLES; GROWTH-FACTOR; CLASS-II; EXOSOMES; MICROVESICLES; TYPE-1; RESPONSES; EXPOSURE
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/25591
- DOI
- 10.1111/J.1398-9995.2010.02359.X
- ISSN
- 0105-4538
- Article Type
- Article
- Citation
- ALLERGY, vol. 65, no. 10, page. 1256 - 1265, 2010-10
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- There are no files associated with this item.
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