Bioconjugation of Hydroxylated Semiconductor Nanocrystals and Background-Free Biomolecule Detection
SCIE
SCOPUS
- Title
- Bioconjugation of Hydroxylated Semiconductor Nanocrystals and Background-Free Biomolecule Detection
- Authors
- Kim, Y; Kim, W; Yoon, HJ; Shin, SK
- Date Issued
- 2010-07
- Publisher
- AMER CHEMICAL SOC
- Abstract
- Semiconductor nanocrystals emerge as fluorescent bioprobes for long-term imaging and multiplexed assays; however, there is a challenge of making nanocrystals biocompatible without nonspecific bindings to background molecules. Here, we report the bioconjugation of small-sized, hydroxylated nanocrystals, enabling highly sensitive detection of various biomolecules with little or no nonspecific binding. Zinc-blende CdSc/ZnS nanocrystals were passivated with 3-mercapto-l-propanol (MPO) and activated to amine-reactive succinimidyl carbonate derivatives and then covalently linked to amine-functionalized biornolecules, such as biotin, DNA, and hemagglutinin peptide, by forming a carbamate linkage. Tris(3-hydroxypropyl)phosphine was added to stabilize the zinc thiolate linkage on nanocrystals. For comparison, CdSe/ZnS nanocrystals were passivated with 3-mercaptopropionic acid (MPA) and conjugated with aminated biomolecules. Photoluininescence properties of organic, water-soluble, and bioconjugated nanocrystals were characterized. Significantly, the bioconjugates of hydroxylated (CdSe/ZnS MPO) nanocrystals exhibited brighter photoluminescence with longer lifetimes than those of carboxylatcd (CdSe/ ZnS MPA) nanocrystals. Specific and nonspecific interactions between nanocrystals and biornolccules were examined by incubating nanocrystal bioconjugates with avidm agarose beads, anti-hemagglutinin affinity in DNA glass slide, or avidin glass slide. CdSe/ZnS MPO nanocrystals showed little or no nonspecific binding to both agarose beads and glass slides, whereas CdSe/ZnS MPA nanocrystals exhibited significant nonspecific binding due to the carboxyl amine interactions. Notably, CdSc/ZnS MPO bioconjugates yield about 20 times brighter images than CdSe/ZnS MPA bioconjugates in both DNA hybridization and biotin streptavidin binding. Hydroxylated nanocrystals stabilized by hydroxyphosphine are small, bright, and photostable in physiological conditions, and their bioconjugates afford background-free detection of specific bimolecular interactions, positioning them for an ideal fluorescent probe to biological settings.
- Keywords
- WATER-SOLUBLE PHOSPHINES; FLUORESCENT QUANTUM DOTS; CDSE NANOCRYSTALS; SURFACE-COATINGS; LIVE CELLS; DNA; PROTEIN; HYBRIDIZATION; PEPTIDE
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/25511
- DOI
- 10.1021/BC100114Q
- ISSN
- 1043-1802
- Article Type
- Article
- Citation
- BIOCONJUGATE CHEMISTRY, vol. 21, no. 7, page. 1305 - 1311, 2010-07
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