Phosphoinositides Regulate Clathrin-dependent endocytosis at the Tip of Pollen Tubes in Arabidopsis Tobacco.
SCIE
SCOPUS
- Title
- Phosphoinositides Regulate Clathrin-dependent endocytosis at the Tip of Pollen Tubes in Arabidopsis Tobacco.
- Authors
- Yan Zhao; An Yan; Jose Feijo; Masahiro Furutani; Tadaomi Takenawa; Hwang, I; Ying Fu; Zhenbiao Yang
- Date Issued
- 2010-12
- Publisher
- ASPB
- Abstract
- Using the tip-growing pollen tube of Arabidopsis thaliana and Nicotiana tabacum as a model to investigate endocytosis mechanisms, we show that phosphatidylinositol-4-phosphate 5-kinase 6 (PIP5K6) regulates clathrin-dependent endocytosis in pollen tubes. Green fluorescent protein-tagged PIP5K6 was preferentially localized to the subapical plasma membrane (PM) in pollen tubes where it apparently converts phosphatidylinositol 4-phosphate (PI4P) to phosphatidylinositol 4,5-bisphosphate [PI(4,5)P-2]. RNA interference-induced suppression of PIP5K6 expression impaired tip growth and inhibited clathrin-dependent endocytosis in pollen tubes. By contrast, PIP5K6 overexpression induced massive aggregation of the PM in pollen tube tips. This PM abnormality was apparently due to excessive clathrin-dependent membrane invagination because this defect was suppressed by the expression of a dominant-negative mutant of clathrin heavy chain. These results support a role for PI(4,5)P-2 in promoting early stages of clathrin-dependent endocytosis (i.e., membrane invagination). Interestingly, the PIP5K6 overexpression-induced PM abnormality was partially suppressed not only by the overexpression of PLC2, which breaks down PI(4,5)P-2, but also by that of PI4K beta 1, which increases the pool of PI4P. Based on these observations, we propose that a proper balance between PI4P and PI(4,5)P-2 is required for clathrin-dependent endocytosis in the tip of pollen tubes.
- Keywords
- PHOSPHATIDYLINOSITOL 4,5-BISPHOSPHATE; ACTIN DYNAMICS; CELL POLARITY; RHO-GTPASE; MEMBRANE TRAFFICKING; NICOTIANA-TABACUM; SEQUENCE-ANALYSIS; CALCIUM INFLUX; COATED PITS; GROWTH
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/25162
- DOI
- 10.1105/tpc.110.076760
- ISSN
- 1040-4651
- Article Type
- Article
- Citation
- PLANT CELL, vol. 22, no. 12, page. 4031 - 4044, 2010-12
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- There are no files associated with this item.
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