Serotonin-induced regulation of the actin network for learning-related synaptic growth requires Cdc42, N-WASP, and PAK in Aplysia sensory neurons
SCIE
SCOPUS
- Title
- Serotonin-induced regulation of the actin network for learning-related synaptic growth requires Cdc42, N-WASP, and PAK in Aplysia sensory neurons
- Authors
- Udo, H; Jin, I; Kim, JH; Li, HL; Youn, T; Hawkins, RD; K; el, ER; Bailey, CH
- Date Issued
- 2005-03-24
- Publisher
- CELL PRESS
- Abstract
- Application of Clostridium difficile toxin B, an inhibitor of the Rho family of GTPases, at the Aplysia sensory to motor neuron synapse blocks long-term facilitation and the associated growth of new sensory neuron varicosities induced by repeated pulses of serotonin (5-HT). We have isolated cDNAs encoding Aplysia Rho, Rac, and Cdc42 and found that Rho and Rac had no effect but that overexpression in sensory neurons of a dominant-negative mutant of ApCdc42 or the CRIB domains of its downstream effectors PAK and N-WASP selectively reduces the long-term changes in synaptic strength and structure. FRET analysis indicates that 5-HT activates ApCdc42 in a subset of varicosities contacting the postsynaptic motor neuron and that this activation is dependent on the PI3K and PLC signaling pathways. The 5-HT-induced activation of ApCdc42 initiates reorganization of the presynaptic actin network leading to the outgrowth of filopodia, some of which are morphological precursors for the learning-related formation of new sensory neuron varicosities.
- Keywords
- LONG-TERM FACILITATION; CELL-CELL ADHESION; RHO GTPASES; STRUCTURAL-CHANGES; DEPOLYMERIZING PROTEIN; P21-ACTIVATED KINASE; DENDRITIC SPINES; MEMORY STORAGE; RAC; PLASTICITY
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/24686
- DOI
- 10.1016/J.NEURON.2005.01.044
- ISSN
- 0896-6273
- Article Type
- Article
- Citation
- NEURON, vol. 45, no. 6, page. 887 - 901, 2005-03-24
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