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Cited 12 time in webofscience Cited 41 time in scopus
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DNA MICROARRAYS ON A DENDRON-MODIFIED SURFACE IMPROVE SIGNIFICANTLY THE DETECTION OF SINGLE NUCLEOTIDE VARIATIONS IN THE P53 GENE

Title
DNA MICROARRAYS ON A DENDRON-MODIFIED SURFACE IMPROVE SIGNIFICANTLY THE DETECTION OF SINGLE NUCLEOTIDE VARIATIONS IN THE P53 GENE
Authors
Oh, SJJu, JMKim, BCKo, EHong, BJPark, JGPark, JWChoi, KY
POSTECH Authors
Park, JWChoi, KY
Date Issued
Jan-2005
Publisher
OXFORD UNIV PRESS
Abstract
Selectivity and sensitivity in the detection of single nucleotide polymorphisms (SNPs) are among most important attributes to determine the performance of DNA microarrays. We previously reported the generation of a novel mesospaced surface prepared by applying dendron molecules on the solid surface. DNA microarrays that were fabricated on the dendron-modified surface exhibited outstanding performance for the detection of single nucleotide variation in the synthetic oligonucleotide DNA. DNA microarrays on the dendron-modified surface were subjected to the detection of single nucleotide variations in the exons 5-8 of the p53 gene in genomic DNAs from cancer cell lines. DNA microarrays on the dendron-modified surface clearly discriminated single nucleotide variations in hotspot codons with high selectivity and sensitivity. The ratio between the fluorescence intensity of perfectly matched duplexes and that of single nucleotide mismatched duplexes was 5-100 without sacrificing signal intensity. Our results showed that the outstanding performance of DNA microarrays fabricated on the dendron-modifled surface is strongly related to novel properties of the dendron molecule, which has the conical structure allowing mesospacing between the capture probes. Our microarrays on the dendron-modified surface can reduce the steric hindrance not only between the solid surface and target DNA, but also among immobilized capture probes enabling the hybridization process on the surface to be very effective. Our DNA microarrays on the dendron-modified surface could be applied to various analyses that require accurate detection of SNPs.
Keywords
TUMOR-SUPPRESSOR GENE; OLIGONUCLEOTIDE PROBE ARRAY; CLINICAL IMPLICATIONS; SEQUENCE-ANALYSIS; HYBRIDIZATION; CANCER; EXPRESSION; MUTATIONS; TP53; EFFICIENCY
URI
http://oasis.postech.ac.kr/handle/2014.oak/24473
DOI
10.1093/NAR/GNI087
ISSN
0305-1048
Article Type
Article
Citation
NUCLEIC ACIDS RESEARCH, vol. 33, no. 10, page. E90, 2005-01
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최관용CHOI, KWAN YONG
Div of Integrative Biosci & Biotech
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