Ultra-sensitive analysis of microcystin LR using microchip based detection system
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KCI
- Title
- Ultra-sensitive analysis of microcystin LR using microchip based detection system
- Authors
- Pyo, D; Huang, Y; Kim, Y; Hahn, JH
- Date Issued
- 2005-06-20
- Publisher
- KOREAN CHEMICAL SOC
- Abstract
- For the detection of cyanobacterial toxin, an Enzyme-linked immunosorbent assay (ELISA) was integrated into a PDMS microchip. The conjugates of microcystin-LR (MCLR) and keyhole limpet hemocyanin (KLH) were adsorbed on the surface of polystyrene beads and these MCLR-KLH polystyrene beads were introduced into a microchamber. MCLR on the surface of polystyrene beads reacted with horseradish peroxides (HRP) conjugated anti-MCLR monoclonal antibody (mAb) which had a competitive reaction with MCLR in water sample. After the enzyme substrate 3,3,5,5-tetramethyl benzidine (TMB) was injected into the chamber and catalyzed by HRP, the color change was detected with a liquid-cord waveguide. This integration shortened the conventional ELISA analysis time from several hours to about 30 min with only 4.2 mu L MCLR sample consuming which was useful for the environmental analysis. More over, troublesome operations required for ELISA could be replaced by simple operations. The microchip based detection system showed a good sensitivity of 0.05 mu g/L and maintained good reliability through its quantitative range with low coefficients of variation (2.5-10.5%).
- Keywords
- microchip; microcystin; sensitive analysis; CAPILLARY-ELECTROPHORESIS; MONOCLONAL-ANTIBODIES; SOFT LITHOGRAPHY; CYANOBACTERIA; HEPATOTOXINS; IMMUNOASSAY; AERUGINOSA; CANCER; ASSAY
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/24447
- DOI
- 10.5012/bkcs.2005.26.6.939
- ISSN
- 0253-2964
- Article Type
- Article
- Citation
- BULLETIN OF THE KOREAN CHEMICAL SOCIETY, vol. 26, no. 6, page. 939 - 942, 2005-06-20
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