Reduction of viral loads by multigenic DNA priming and adenovirus boosting in the SIVmac-macaque model
SCIE
SCOPUS
- Title
- Reduction of viral loads by multigenic DNA priming and adenovirus boosting in the SIVmac-macaque model
- Authors
- Suh, YS; Park, KS; Sauermann, U; Franz, M; Norley, S; Wilfingseder, D; Stoiber, H; Fagrouch, Z; Heeney, J; Hunsmann, G; Stahl-Hennig, C; Sung, YC
- Date Issued
- 2006-03-10
- Publisher
- ELSEVIER SCI LTD
- Abstract
- In this study, we investigated the ability of a multigenic SIV DNA prime/replication-defective adenovirus serotype 5 (rAd/SIV) boost regimen to induce SIV-specific immune responses and protection against intrarectal challenge with SIVmac251 in rhesus macaques. Four rhesus macaques were immunized intramuscularly three times at 8-week intervals with SIV DNA vaccine and boosted once with rAd/STV vaccine Four control macaques received the same amount of mock plasmid DNA and mock adenovirus vector. While the SIV DNA vaccine included plasmids expressing a mutated human IL-12 gene (IL-12N222L) as well as SIVmac239 structural and regulatory genes, the rAd/SIV vaccine contained rAd vectors expressing SIVmac239 genes only. Immunization with SIV DNA vaccine alone induced SIV-specific IFN-gamma ELISPOT responses in only two of four vaccinated macaques, whereas all animals developed SIV-specific T-cell responses and Env- and Tat-specific antibody responses following the rAd/SIV vaccine boost. Upon intrarectal challenge with pathogenic SIVmac251, strong anamnestic Env-specific binding and neutralizing antibody responses were detected in the vaccinated macaques. Overall, the immunized macaques had lower peak and set-point viral loads than control macaques, suggesting that the induced immune responses play a role in the control of viremia. In addition. the loss of CD4(+) T cells was delayed in the vaccinated macaques after challenge. These results indicate that the multigenic DNA prime-adenovirus boost immunization may be a promising approach in developing an effective AIDS vaccine. (c) 2005 Elsevier Ltd. All rights reserved.
- Keywords
- SIV; multigenic vaccine; prime-boost; IL-12; SIMIAN IMMUNODEFICIENCY VIRUS; T-CELL RESPONSES; INFECTED RHESUS-MONKEYS; NEUTRALIZING ANTIBODIES; IMMUNE-RESPONSES; DISEASE PROGRESSION; MUCOSAL CHALLENGE; VACCINIA VIRUS; OPTIMAL INDUCTION; PROTEIN VACCINE
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/24125
- DOI
- 10.1016/j.vaccine.2005.10.026
- ISSN
- 0264-410X
- Article Type
- Article
- Citation
- VACCINE, vol. 24, no. 11, page. 1811 - 1820, 2006-03-10
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