Proteomic pattern-based analyses of light responses in Arabidopsis thaliana wild-type and photoreceptor mutants
SCIE
SCOPUS
- Title
- Proteomic pattern-based analyses of light responses in Arabidopsis thaliana wild-type and photoreceptor mutants
- Authors
- Kim, DS; Cho, DS; Park, WM; Na, HJ; Nam, HG
- Date Issued
- 2006-05
- Publisher
- WILEY-V C H VERLAG GMBH
- Abstract
- Light critically affects the physiology of plants. Using two-dimensional gel electrophoresis, we used a proteomics approach to analyze the responses of Arabidopsis thaliana to red (660 nm), far-red (730 nm) and blue (450 nm) light, which are utilized by type II and type I phytochromes, and blue light receptors, respectively. Under specific light treatments, the proteomic profiles of 49 protein spots exhibited over 1.8-fold difference in protein abundance, significant at p < 0.05. Most of these proteins were metabolic enzymes, indicating metabolic changes induced by light of specific wavelengths. The differentially-expressed proteins formed seven dusters, reflecting coregulation. We used the 49 differentially-regulated proteins as molecular markers for plant responses to light, and by developing a procedure that calculates the Pearson correlation distance of cluster-to-cluster similarity in expression changes, we assessed the proteome-based relatedness of light responses for wild-type and phytochrome mutant plants. Overall, this assessment was consistent with the known physiological responses of plants to light. However, we also observed a number of novel responses at the proteomic level, which were not predicted from known physiological changes.
- Keywords
- Arabidopsis thaliana; cluster analysis; light; Pearson distance; proteome; SIGNAL-TRANSDUCTION; GENOME EXPRESSION; MASS-SPECTROMETRY; GENE-EXPRESSION; PHYTOCHROME; IDENTIFICATION; PROTEIN; ELECTROPHORESIS; SEQUENCES; PATHWAYS
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/24001
- DOI
- 10.1002/PMIC.2005006
- ISSN
- 1615-9853
- Article Type
- Article
- Citation
- PROTEOMICS, vol. 6, no. 10, page. 3040 - 3049, 2006-05
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