Rice bending lamina 2 (bla2) mutants are defective in a cytochrome P450 (CYP734A6) gene predicted to mediate brassinosteroid catabolism
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- Title
- Rice bending lamina 2 (bla2) mutants are defective in a cytochrome P450 (CYP734A6) gene predicted to mediate brassinosteroid catabolism
- Authors
- Park, W; Kim, HB; Kim, WT; Park, PB; An, G; Choe, S
- Date Issued
- 2006-12-31
- Publisher
- BOTANICAL SOCIETY OF KOREA
- Abstract
- Desirable morphological traits in rice plants, such as shorter stalks and erect leaves, are being pursued in breeding programs. Rice brassinosteroid (BR)-deficient mutants display the phenotype of reduced heights and erect lamina. Therefore, biotechnology can be used for controlled deactivation of bioactive BRs in specific tissues. Here, we isolated a gene encoding for a BR-deactivating enzyme. Based on its sequence identity with a known enzyme, Arabidopsis Cytochrome P450 734A1, we identified a rice homolog named OsCYP734A6 (LOC_Os01g29150). A search of the rice T-DNA mutant population for insertional mutants of this gene revealed two alleles. These T-DNA loss-of-function mutants displayed a strongly bending lamina phenotype similar to the morphology of rice plants treated with BRs. These two mutants were named bending lamina (bla)2-1 and bla2-2. In Arabidopsis, the transcript level of a putative BR biosynthetic gene, OsDWARF4, is feed-back down-regulated in response to exogenous application of BRs. Similarly, a steady-state level of OsDWARF4 transcripts was significantly down-regulated here in the bla2 mutant background. Based on their sequence similarity, the bending-lamina phenotype in the bla2 mutant, and down-regulation of the BR biosynthetic gene OsDWARF4, we therefore propose that OsCYP734A6 (BLA2) is likely to be involved in BR deactivation.
- Keywords
- brassinolide; cytochrome P450; erect leaves; rice T-DNA mutant; short stature; ARABIDOPSIS; ACTIVATION; EXPRESSION; CYP72C1; DWARF4; YIELD
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/23621
- DOI
- 10.1007/BF03031128
- ISSN
- 1226-9239
- Article Type
- Article
- Citation
- JOURNAL OF PLANT BIOLOGY, vol. 49, no. 6, page. 469 - 476, 2006-12-31
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