Self-duplex formation of an A(Py)-substituted oligodeoxyadenylate and its unique fluorescence
SCIE
SCOPUS
- Title
- Self-duplex formation of an A(Py)-substituted oligodeoxyadenylate and its unique fluorescence
- Authors
- Seo, YJ; Rhee, H; Joo, T; Kim, BH
- Date Issued
- 2007-04-25
- Publisher
- AMER CHEMICAL SOC
- Abstract
- Unexpected homoadenine self-duplexes are formed when pyrene units are bound covalently to the deoxyadenosine bases at specific distances (1,4 relationships). This discovery illustrates how small-molecule pyrene intercalators can be used to drive unknown nucleic acid assembly with a concomitant change in fluorescence. When a pair of pyrene fluorophore units is located within an oligodeoxyadenylate chain, the system can display three different colors (reddish-orange, green, or blue) depending on the relative location of the fluorophores. A unique fluorescence signal, a reddish band peaking at 580 nm, appears when the oligomers possess more than two spacers between the pyrene fluorophores(1,4 relationships). Several spectroscopic experiments, for example, recording variable-concentration spectra, CD, UV, melting temperature, and gel electropherogram, indicate that this new reddish band came from an intermolecular homoadenine self-duplex. Time-resolved fluorescence measurements using both TCSPC and upconversion methods indicate that this unique fluorescence has a long lifetime.
- Keywords
- FREE MOLECULAR BEACON; QUENCHER-FREE; NUCLEIC-ACIDS; DNA; STACKING; BINDING; OLIGONUCLEOTIDES; FLUOROPHORES; AGGREGATION; STABILITY
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/23444
- DOI
- 10.1021/JA069069I
- ISSN
- 0002-7863
- Article Type
- Article
- Citation
- JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, vol. 129, no. 16, page. 5244 - 5247, 2007-04-25
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