Fast size-exclusion chromatography at high temperature
SCIE
SCOPUS
- Title
- Fast size-exclusion chromatography at high temperature
- Authors
- Park, S; Cho, HS; Kim, Y; Ahn, S; Chang, TY
- Date Issued
- 2007-07-20
- Publisher
- ELSEVIER SCIENCE BV
- Abstract
- We report on the size-exclusion chromatography (SEC) operated at high column temperature to reduce the analysis time. The column temperature was raised beyond the normal boiling point of the eluent and a sufficient column backpressure was applied to prevent the mobile phase from boiling by inserting a narrow bore tubing between the separation column and the detector. The narrow bore tubing also functions to cool the effluent down to the room temperature before it reaches the detector. Therefore, normal SEC detectors can be used without any modification. It was confirmed that the SEC analysis time could be shortened significantly by the high-temperature operation without serious deterioration in the resolution. (c) 2007 Elsevier B.V. All rights reserved.
- Keywords
- high temperature; size-exclusion chromatography; fast analysis; 2-DIMENSIONAL LIQUID-CHROMATOGRAPHY; GEL-PERMEATION CHROMATOGRAPHY; ELEVATED-TEMPERATURE; SEPARATION; POLYMERS; HPLC
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/23289
- DOI
- 10.1016/j.chroma.2007.04.049
- ISSN
- 0021-9673
- Article Type
- Article
- Citation
- JOURNAL OF CHROMATOGRAPHY A, vol. 1157, no. 39815, page. 96 - 100, 2007-07-20
- Files in This Item:
- There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.