Phospholipase C-gamma 1 potentiates integrin-dependent cell spreading and migration through Pyk2/paxillin activation
SCIE
SCOPUS
- Title
- Phospholipase C-gamma 1 potentiates integrin-dependent cell spreading and migration through Pyk2/paxillin activation
- Authors
- Choi, JH; Yang, YR; Lee, SK; Kim, IS; Ha, SH; Kim, EK; Bae, YS; Ryu, SH; Suh, PG
- Date Issued
- 2007-08
- Publisher
- ELSEVIER SCIENCE INC
- Abstract
- Phospholipase C-gamma 1 I (PLC-gamma 1), which generates two second messengers, namely, inositol-1,4,5-trisphosphate and diacylglycerol, is implicated in growth factor-mediated chemotaxis. However, the exact role of PLC-gamma 1 in integrin-mediated cell adhesion and migration remains poorly understood. In this study, we demonstrate that PLC-gamma 1 is required for actin cytoskeletal organization and cell motility through the regulation of Pyk2 and paxillin activation. After fibronectin stimulation, PLC-gamma 1 directly interacted with the cytoplasmic tail of integrin beta 1. In PLC-gamma 1-silenced cells, integrin-induced Pyk2 and paxillin phosphorylation were significantly reduced and PLC-gamma 1 potentiated the integrin-induced Pyk2/paxillin activation in its enzymatic activity-dependent manner. In addition, specific knock-down of PLC-gamma 1 resulted in a failure to form focal adhesions dependent on fibronectin stimulation, which appeared to be caused by the suppression of Pyk2 and paxillin phosphorylation. Interestingly, PLC-gamma 1 potentiated the activations of Rac, thus integrin-induced lamellipodia formation was up-regulated. Consequently, the strength of cell-substratum interaction and cell motility were profoundly up-regulated by PLC-gamma 1. Taken together, these results suggest that PLC-gamma 1 is a key player in integrin-mediated cell spreading and motility achieved by the activation of Pyk2/paxillin/Rac signaling. (c) 2007 Elsevier Inc. All rights reserved.
- Keywords
- phospholipase C-gamma l; pyk2; paxillin; rac1; integrin; adhesion; migration; FOCAL ADHESION KINASE; RICH TYROSINE KINASE-2; GROWTH-FACTOR RECEPTOR; ACTIN STRESS FIBERS; C-GAMMA-L; DIFFERENTIAL REGULATION; INVASION; PHOSPHORYLATION; MOTILITY; RAC
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/23269
- DOI
- 10.1016/j.cellsig.2007.04.002
- ISSN
- 0898-6568
- Article Type
- Article
- Citation
- CELLULAR SIGNALLING, vol. 19, no. 8, page. 1784 - 1796, 2007-08
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