Enhanced benzophenanthridine alkaloid production and protein expression with combined elicitor in Eschscholtzia californica suspension cultures
SCIE
SCOPUS
- Title
- Enhanced benzophenanthridine alkaloid production and protein expression with combined elicitor in Eschscholtzia californica suspension cultures
- Authors
- Cho, HY; Lee-Parsons, CWT; Yoon, SYH; Rhee, HS; Park, JM
- Date Issued
- 2007-12
- Publisher
- SPRINGER
- Abstract
- Production of the benzophenanthridine alkaloids in Eschscholtzia californica suspension cell cultures was optimized by adding 0.5 mg methyl jasmonate (MJ) and 0.02 mg salicylic acid (SA)/g FCW after 7 days cultivation. Sanguinarine reached 24 mg/g DCW by such treatment; 10 times higher than in control cell cultures. MJ and SA induced expression of berberine bridge enzyme and 3'-hydroxy-(S)-N-methylcoclaurine-4'-O-methyltransferase, respectively. MJ plus SA induced over-expression of both enzymes.
- Keywords
- benzophenanthridine alkaloid; Eschscholtzia californica; methyl jasmonate; salicylic acid; signal transduction; Western blot; CELL-CULTURES; METHYL JASMONATE; MOLECULAR CHARACTERIZATION; SECONDARY METABOLITES; DEFENSE RESPONSES; BIOSYNTHESIS; ELICITATION; ACCUMULATION; ARABIDOPSIS; RESISTANCE
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/23100
- DOI
- 10.1007/s10529-007-9469-4
- ISSN
- 0141-5492
- Article Type
- Article
- Citation
- BIOTECHNOLOGY LETTERS, vol. 29, no. 12, page. 2001 - 2005, 2007-12
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