Label-free detection of DNA molecules on the dendron based self-assembled monolayer by electrochemical impedance spectroscopy

Abstract

We report preparation of a novel platform for effective DNA hybridization and its application to the detection of single mismatched DNA. Cone-shaped dendrimer molecules have been immobilized on the gold surface at equidistance, 3.1 nm, from each other with a probe DNA molecule attached to the top of each dendrimer so that enough space would be secured for effective hybridization. This arrangement allows each probe DNA molecule to form a natural DNA double helix upon hybridization with a target DNA molecule. The single nucleotide polymorphism at either the central or end position of the 25-mer target DNA has been shown to be effectively discriminated against on this platform from each other as well as from a complementary DNA by electrochemical impedance measurements. We also report adverse effects exerted by probe ions, Fe(CN)(6)(3-/4-), on DNA hybridization reactions. The significance of the results for the use in DNA analysis is discussed. (C) 2008 Elsevier B.V. All rights reserved.