Label-free detection of DNA molecules on the dendron based self-assembled monolayer by electrochemical impedance spectroscopy
- Title
- Label-free detection of DNA molecules on the dendron based self-assembled monolayer by electrochemical impedance spectroscopy
- Authors
- Park, JY; Kwon, SH; Park, JW; Park, SM; 박수문
- Date Issued
- 2008-06
- Publisher
- ELSEVIER SCIENCE BV
- Abstract
- We report preparation of a novel platform for effective DNA hybridization and its application to the detection of single mismatched DNA. Cone-shaped dendrimer molecules have been immobilized on the gold surface at equidistance, 3.1 nm, from each other with a probe DNA molecule attached to the top of each dendrimer so that enough space would be secured for effective hybridization. This arrangement allows each probe DNA molecule to form a natural DNA double helix upon hybridization with a target DNA molecule. The single nucleotide polymorphism at either the central or end position of the 25-mer target DNA has been shown to be effectively discriminated against on this platform from each other as well as from a complementary DNA by electrochemical impedance measurements. We also report adverse effects exerted by probe ions, Fe(CN)(6)(3-/4-), on DNA hybridization reactions. The significance of the results for the use in DNA analysis is discussed. (C) 2008 Elsevier B.V. All rights reserved.
- Keywords
- self-assembled monolayer of dendrimer molecules; monodispersed self-assembled monolayer; electrochemical impedance spectroscopy; spacer; dioxyribonucleic acid analysis; FOURIER-TRANSFORM; HYBRIDIZATION; TEMPLATES; POLYMERS; MICROARRAYS; ELECTRODES; SENSORS; SURFACE; GROWTH
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/22673
- DOI
- 10.1016/J.ACA.2008.0
- ISSN
- 0003-2670
- Article Type
- Conference
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- There are no files associated with this item.
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