Biochemical indication of microbial mass changes using ATP and DNA measurement in biological treatment of thiocyanate
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SCOPUS
- Title
- Biochemical indication of microbial mass changes using ATP and DNA measurement in biological treatment of thiocyanate
- Authors
- Lim, J; Lee, S; Kim, SD; Hwang, S
- Date Issued
- 2008-09
- Publisher
- SPRINGER
- Abstract
- This study was designed to monitor changes in the levels of adenosine 5'-triphosphate (ATP) and deoxyribonucleic acid (DNA) per unit of microbial mass during the autotrophic biodegradation of thiocyanate (SCN(-)). An artificial medium containing trace minerals and 500 mg SCN(-)/L was used as a substrate for bacterial growth. An SCN(-)-degrading bioreactor with a working volume of 6 L, equipped with temperature, pH, and dissolved oxygen controls, was operated in batch mode. During the exponential phase of SCN(-) biodegradation, the ratios of ATP and DNA to microbial dry weight varied from 0.6 to 1.1 mu g ATP/mg of volatile suspended solid (VSS), and from 3.5 to 8.8 mu g DNA/mg of VSS, respectively. The ATP and DNA concentrations correlated linearly with microbial mass (r(2) > 0.9) within the exponential phase. The linear regression equations were as follows: (1) microbial mass concentration (mg/L) = 0.663 x ATP concentration (mu g/L) + 11.1 and (2) microbial concentration (mg/L) = 0.081 x DNA concentration (mu g/L) + 10.9. The applicable ranges were 6.8 to 47.4 mu g/L for ATP concentration and 41.5 to 395 mu g/L for DNA concentration, respectively.
- Keywords
- ATP; DNA; microbial mass; thiocyanate biodegradation; WASTE-WATER; FLOW-CYTOMETRY; CELL-DIVISION; BIOMASS; BIODEGRADATION; ADENOSINE; GROWTH; ACIDOGENESIS; OPTIMIZATION; TRIPHOSPHATE
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/22553
- DOI
- 10.1007/s00253-008-1601-4
- ISSN
- 0175-7598
- Article Type
- Article
- Citation
- APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, vol. 80, no. 3, page. 525 - 530, 2008-09
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