Sustained CPEB-dependent local protein synthesis is required to stabilize synaptic growth for persistence of long-terra facilitation in Aplysia
SCIE
SCOPUS
- Title
- Sustained CPEB-dependent local protein synthesis is required to stabilize synaptic growth for persistence of long-terra facilitation in Aplysia
- Authors
- Miniaci, MC; Kim, JH; Puthanveettil, SV; Si, K; Zhu, HX; K; el, ER; Bailey, CH
- Date Issued
- 2008-09-25
- Publisher
- CELL PRESS
- Abstract
- The time course of the requirement for local protein synthesis in the stabilization of learning-related synaptic growth and the persistence of long-term memory was examined using Aplysia bifurcated sensory neuron-motor neuron cultures. We find that, following repeated pulses of serotonin (5-HT), the local perfusion of emetine, an inhibitor of protein synthesis, or a TAT-AS oligonucleotide directed against ApCPEB blocks long-term facilitation (LTF) at either 24 or 48 hr and leads to a selective retraction of newly formed sensory neuron varicosities induced by 5-HT. By contrast, later inhibition of local protein synthesis, at 72 hr after 5-HT, has no effect on either synaptic growth or LTF. These results define a specific stabilization phase for the storage of long-term memory during which newly formed varicosities are labile and require sustained CPEB-dependent local protein synthesis to acquire the more stable properties of mature varicosities required for the persistence of LTF.
- Keywords
- SENSORY NEURONS; MEMORY STORAGE; STRUCTURAL-CHANGES; WITHDRAWAL REFLEX; GILL-WITHDRAWAL; IN-VIVO; PLASTICITY; SYNAPSES; SENSITIZATION; TRANSIENT
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/22472
- DOI
- 10.1016/J.NEURON.2008.07.036
- ISSN
- 0896-6273
- Article Type
- Article
- Citation
- NEURON, vol. 59, no. 6, page. 1024 - 1036, 2008-09-25
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- There are no files associated with this item.
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