Phospholipase A₂β mediates light-induced stomatal opening in Arabidopsis
SCIE
SCOPUS
- Title
- Phospholipase A₂β mediates light-induced stomatal opening in Arabidopsis
- Authors
- Seo, J; Lee, HY; Choi, H; Choi, Y; Lee, Y; Kim, YW; Ryu, SB; Lee, Y
- Date Issued
- 2008-10
- Publisher
- OXFORD UNIV PRESS
- Abstract
- Phospholipase A(2) (PLA(2)) catalyses the hydrolysis of phospholipids into lysophospholipids and free fatty acids. Physiological studies have indicated that PLA(2) is involved in stomatal movement. However, genetic evidence of a role of PLA(2) in guard cell signalling has not yet been reported. To identify PLA(2) gene(s) that is (are) involved in light-induced stomatal opening, stomatal movement was examined in Arabidopsis thaliana plants in which the expression of PLA(2) isoforms was reduced or knocked-out. Light-induced stomatal opening in PLA(2)alpha knockout plants did not differ from wild-type plants. Plants in which PLA(2)beta was silenced by RNA interference exhibited delayed light-induced stomatal opening, and this phenotype was reversed by exogenous lysophospholipids, which are products of PLA(2). Stomatal opening in transgenic plants that over-expressed PLA(2)beta was faster than wild-type plants. The expression of PLA(2)beta was localized to the endoplasmic reticulum of guard cells, and increased in response to light in the mature leaf. Aristolochic acid, which inhibits light-induced stomatal opening, inhibited the activity of purified PLA(2)beta. Collectively, these results provide evidence that PLA(2)beta is involved in light-induced stomatal opening in Arabidopsis.
- Keywords
- Guard cell; light signal transduction; phospholipase A(2); stomata; MEMBRANE H+-ATPASE; GUARD-CELL PROTOPLASTS; FREE FATTY-ACIDS; PLASMA-MEMBRANE; BLUE-LIGHT; SIGNAL-TRANSDUCTION; PROTEIN; ACTIVATION; BINDING; PLANTS
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/22455
- DOI
- 10.1093/JXB/ERN208
- ISSN
- 0022-0957
- Article Type
- Article
- Citation
- JOURNAL OF EXPERIMENTAL BOTANY, vol. 59, no. 13, page. 3587 - 3594, 2008-10
- Files in This Item:
- There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.