Use of order-specific primers to investigate the methanogenic diversity in acetate enrichment system
SCIE
SCOPUS
- Title
- Use of order-specific primers to investigate the methanogenic diversity in acetate enrichment system
- Authors
- Shin, SG; Lee, CS; Hwang, K; Ahn, JH; Hwang, S
- Date Issued
- 2008-11
- Publisher
- SPRINGER HEIDELBERG
- Abstract
- The applicability of order-specific primers in minimizing the possible underestimation of microbial diversity was evaluated via denaturing gradient gel electrophoresis (DGGE) analysis of a lab-scale anaerobic digester. Initially, a population analysis with real-time quantitative PCR demonstrated the existence of three methanogenic orders-Methanobacteriales, Methanomicrobiales, and Methanosarcinales-throughout the reaction period. DGGE analyses with three pairs of order-specific primers yielded eight operational taxonomic units (OTUs), whereas DGGE analysis with two independent Archaea-specific primers identified only five. Moreover, the order-specific primers amplified at least one OTU affiliated with each order, whereas no members of Methanobacteriales or Methanomicrobiales were identified with Archaea-specific primers in most samples. These findings provide evidence that order-specific analysis can detect the diversity of methanogens in greater detail than conventional Archaea-specific analysis.
- Keywords
- Diversity of methanogens; Taxonomic level; Order-specific primers; Real-time quantitative PCR; Denaturing gradient gel electrophoresis; POLYMERASE-CHAIN-REACTION; 16S RIBOSOMAL-RNA; REAL-TIME PCR; GROUP-SPECIFIC QUANTIFICATION; GRADIENT GEL-ELECTROPHORESIS; MICROBIAL COMMUNITY; START-UP; ANAEROBIC BIOREACTOR; POPULATION-DYNAMICS; PROBE SETS
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/22451
- DOI
- 10.1007/s10295-008-0417-7
- ISSN
- 1367-5435
- Article Type
- Article
- Citation
- JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY, vol. 35, no. 11, page. 1345 - 1352, 2008-11
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