A G-PROTEIN-COUPLED 130-KDA PHOSPHOLIPASE-C ISOZYME, PLC-BETA-4, FROM THE PARTICULATE FRACTION OF BOVINE CEREBELLUM
SCIE
SCOPUS
- Title
- A G-PROTEIN-COUPLED 130-KDA PHOSPHOLIPASE-C ISOZYME, PLC-BETA-4, FROM THE PARTICULATE FRACTION OF BOVINE CEREBELLUM
- Authors
- KIM, Y; LEE, YH; MIN, DS; RYU, SH; SUH, PG
- Date Issued
- 1993-09-27
- Publisher
- ELSEVIER SCIENCE BV
- Abstract
- A 130 kDa PLC isozyme was purified from the particulate fraction of bovine cerebellum. This PLC was recognized by a polyclonal antiserum generated against the purified 97 kDa PLC-beta4. Reconstitution of the purified 130 kDa PLC with the membranes of C6 Bu-1 cells in the presence of GTPgammaS or AlF4- resulted in PLC activation as well as the association of PLC with the membranes. Both the association and activation were revoked when the membrane was washed with 2 M KCl. The 97 kDa PLC-beta4 did not associate with membranes. These data suggest that the 130 kDa PLC is the intact form of PLC-beta4 the activity of which is likely to be regulated by a G-protein on the membrane.
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/22043
- DOI
- 10.1016/0014-5793(93)80293-4
- ISSN
- 0014-5793
- Article Type
- Article
- Citation
- FEBS LETTERS, vol. 331, no. 1-2, page. 38 - 42, 1993-09-27
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- There are no files associated with this item.
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