ISOLATION AND CHARACTERIZATION OF 2 CDNA CLONES THAT ARE RAPIDLY INDUCED DURING THE WOUND RESPONSE OF ARABIDOPSIS-THALIANA

Abstract

Differential screening of a cDNA library of Arabidopsis thaliana constructed from the plant tissues harvested 1 h after wounding resulted in isolation of 2 wound-inducible cDNA clones. Kinetic analysis revealed that the corresponding genes are rapidly induced upon wounding. Expression of these clones reached the maximum level around 1 - 1.5 h after wounding and then were progressively reduced. The time by which expression returned to the control level was around 3 h after wounding. Partial sequence analysis revealed that the two clones are highly homologous to the S adenosylmethionine synthetase and the glutathione-S-transferase gene, respectively.