TRANSCRIPTIONAL REGULATION OF PHOSPHOLIPASE C-GAMMA-1 GENE DURING MUSCLE DIFFERENTIATION

Abstract

Phospholipase C (PLC) has been known to be a key effector protein in signal transduction pathway for cell proliferation and differentiation. Here, we report the transcriptional regulation of PLC-gamma 1 during myogenic differentiation. Both PLC-gamma 1 mRNA and protein levels were increased during myogenesis. The activity of PLC-gamma 1 was also comparable to the amount estimated by Western blot. Comparing promoter activities of various PLC-gamma 1 gene upstream regions in myoblast and myotube, the responsible region was mapped between -551 and -480. From these results, we conclude that the transcription of PLC-gamma 1. is increased by the control of GPE1 region of PLC-gamma 1 gene during myogenesis. (C) 1995 Academic Press, Inc.