Inhibition of PDGF-induced phosphoinositide-turnover by glucopiericidin A
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SCOPUS
- Title
- Inhibition of PDGF-induced phosphoinositide-turnover by glucopiericidin A
- Authors
- Ahn, SC; Kim, BY; Park, CS; Lee, HS; Suh, PG; Ryu, SH; Rho, HM; Rhee, JS; Mheen, TI; Ahn, JS
- Date Issued
- 1995-09
- Publisher
- ACADEMIC PRESS AUST
- Abstract
- In the search for a substance which would specifically block a particular step in the signal transduction cascade, we identified glucopiericidin A produced by Streptomyces sp, as an inhibitor of phosphoinositide (PI)-turnover in phospholipase-C gamma 1 (PLC-gamma 1) overexpressing NM 3T3 fibroblasts (NIH 3T3 gamma 1). Glucopiericidin A inhibited the formation of inositol phosphate (IPt) in platelet-derived growth factor (PDGF)-stimulated NIH 3T3 gamma 1 cells with an IC50 of 5.0 mu M, In vitro enzyme assay showed the compound had no inhibitory effect on PLC-gamma 1 even at 100 mu M concentration. Glucopiericidin A reduced PDGF-induced tyrosine phosphorylations of proteins, including PDGF receptor and PLC-gamma 1, in the cells. In contrast, glucopiericidin A showed only a slight inhibitory effect on epidermal growth factor (EGF)- (EGF)-induced IPt production and protein tyrosine phosphorylations in A431 cells. These results suggest that glucopiericidin A inhibits PDGF-induced activation of PLC-gamma 1 by reducing the tyrosine kinase activity of the PDGF receptor and it more potently inhibits PI-turnover induced by PDGF than by EGF.
- Keywords
- PDGF; EGF; phosphoinositide-turnover; phospholipase C gamma 1; glucopiericidin A; PHOSPHOLIPASE-C-GAMMA; 2ND MESSENGERS; GROWTH-FACTOR; MITOGENESIS; ANTIBODIES; SUBSTRATE; C-GAMMA-1; RECEPTOR; CELLS
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/21665
- ISSN
- 1039-9712
- Article Type
- Article
- Citation
- BIOCHEMISTRY AND MOLECULAR BIOLOGY INTERNATIONAL, vol. 37, no. 1, page. 125 - 132, 1995-09
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