The promoter activity of the phospholipase C-gamma 2 gene is regulated by a cell-type-specific control element
SCIE
SCOPUS
- Title
- The promoter activity of the phospholipase C-gamma 2 gene is regulated by a cell-type-specific control element
- Authors
- Lee, SJ; Bahk, YY; Yun, DH; Lee, HJ; Lee, YH; Ryu, SH; Suh, PG
- Date Issued
- 1997-04
- Publisher
- MARY ANN LIEBERT INC PUBL
- Abstract
- We have cloned and characterized a genomic DNA spanning the 5'-flanking region, the first and second exons, and the first intron of the human PLC-gamma 2 gene. The proximal upstream region is highly CC-rich and lacks a TATA box, whereas the distal region contains several AT-rich tracts. Multiple transcription initiation sites were identified by primer extension analysis, Based on the transient transfection assays, the major transcriptional activation element was identified between -183 and +43 (G2SE) and a transcriptional repressive element was found between -303 and -184 (G2RE). The expression of PLC-gamma 2 in various cell lines was examined using monoclonal anti-PLC-gamma 2 antibody, PLC-gamma 2 was highly expressed in B-cell lines such as Daudi, SP2, and Ramos cells, whereas it existed at very low levels in Jurkat, 3T3-L1, NBL-7, and C6Bu-1 cells. Moderate levels of PLC-gamma 2 were also detected in C2C12, P19, U937, HL60, A431, and PC12 cells. The 4-kb genomic fragment upstream of -1,654 was able to activate transcription from the PLC-gamma 2 promoter in Daudi and C2C12 cells, but not in Jurkat cells, which is consistent with the PLC-gamma 2 protein expression levels in those cell lines. These results suggest that the cell-type-specific expression of PLC-gamma 2 might be attributed to the transcriptional regulation by the upstream cis-element.
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/21318
- DOI
- 10.1089/dna.1997.16.485
- ISSN
- 1044-5498
- Article Type
- Article
- Citation
- DNA AND CELL BIOLOGY, vol. 16, no. 4, page. 485 - 492, 1997-04
- Files in This Item:
- There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.