Protective immunity against heterologous challenge with encephalomyocarditis virus by VP1 DNA vaccination: effect of coinjection with a granulocyte-macrophage colony stimulating factor gene
SCIE
SCOPUS
- Title
- Protective immunity against heterologous challenge with encephalomyocarditis virus by VP1 DNA vaccination: effect of coinjection with a granulocyte-macrophage colony stimulating factor gene
- Authors
- Sin, JI; Sung, JH; Suh, YS; Lee, AH; Chung, JH; Sung, YC
- Date Issued
- 1997-12
- Publisher
- ELSEVIER SCI LTD
- Abstract
- For DNA vaccination studies, recombinant VP1 protein of encephalomyocarditis virus (EMCV) was produced from Escherichia coli, and eukaryotic VP1 expression vector pCT-Gs-VP1, was generated and used as a DNA vaccine, Mice were immunized intramuscularly (i.m.) with pCT-Gs-VP1 in the presence or absence of plasmid DNA expressing granulocyte-macrophage colony stimulating factor (GM-CSF), and were subsequently analyzed for their anti-VP1 immune responses with recombinant VPI in ELISA, Immunization of mice with pCT-Gs-VP1 resulted in I ipl-specific immune response and 43% protection from subsequent lethal heterologous challenge of EMCV. Coinjection of mice with pCT-Gs-VP1 and plasmid DNA encoding GM-CSF was shown to increase the seroconversion rate of the immunized mice with a single DNA injection, and enhanced to a higher degree VP1-specific immunity, which appeared to result in better protection (about 80%) from lethal virus challenge. Thus, our results provide evidence for the potential use of CM-CSF to induce better immune response and resistance against viral infection in DNA vaccination. (C) 1997 Elsevier Science Ltd.
- Keywords
- DNA vaccination; EMCV; GM-CSF; SKELETAL-MUSCLE; PLASMID DNA; D-VARIANT; RESPONSES; MICE; IMMUNIZATION; PRODUCTS; PROTEIN; ANTIGEN; INVIVO
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/21159
- DOI
- 10.1016/S0264-410X(97)88856-1
- ISSN
- 0264-410X
- Article Type
- Article
- Citation
- VACCINE, vol. 15, no. 17-18, page. 1827 - 1833, 1997-12
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