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Localization of two forms of phospholipase C-beta 1, a and b, in C6Bu-1 cells

Title
Localization of two forms of phospholipase C-beta 1, a and b, in C6Bu-1 cells
Authors
Bahk, YYSong, HBaek, SHPark, BYKim, HRyu, SHSuh, PG
POSTECH Authors
Ryu, SH
Date Issued
Jan-1998
Publisher
ELSEVIER SCIENCE BV
Abstract
Phospholipase C-beta 1 (PLC-beta 1), one of the PLC-beta isozymes, exists as two immunologically distinguishable polypeptides of 150 (PLC-beta 1a) and 140 kDa (PLC-beta 1b) which are encoded in two distinct transcripts and generated by alternative splicing of a single gene, In this study, the subcellular localization of the two phospholipases C-beta 1 proteins was examined in rat CGBu-1 glioma cells using immunological techniques, Immunoblot analysis revealed that the two forms of PLC-beta 1 were detectible in both cytosolic and nuclear fractions, PLC-beta 1a appeared to be located preferentially in the cytosol, whereas PLC-beta 1b was found predominantly in the nuclei of C6Bu-1 cells, Immunocytochemical experiments confirmed the differential localization of the two PLC-beta 1 species in C6Bu-1 cells. These results suggest that the two PLC-beta 1 proteins may have different physiological roles in the cell. (C) 1998 Elsevier Science B.V.
Keywords
phospholipase C-beta 1; C6Bu-1 cell; immunocytochemistry; nuclear localization; PROTEIN-KINASE-C; SWISS 3T3 CELLS; BETA-GAMMA-SUBUNITS; RAT-LIVER NUCLEI; GROWTH FACTOR-I; ACTIVATION; STIMULATION; ISOFORM; PHOSPHORYLATION; DIFFERENTIATION
URI
http://oasis.postech.ac.kr/handle/2014.oak/20916
ISSN
0005-2760
Article Type
Article
Citation
BIOCHIMICA ET BIOPHYSICA ACTA-LIPIDS AND LIPID METABOLISM, vol. 1389, no. 1, page. 76 - 80, 1998-01
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류성호RYU, SUNG HO
Dept of Life Sciences
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