Characterization of Mas-7-induced pore formation in SK-N-BE(2)C human neuroblastoma cells
SCIE
SCOPUS
- Title
- Characterization of Mas-7-induced pore formation in SK-N-BE(2)C human neuroblastoma cells
- Authors
- Suh, BC; Lee, IS; Chae, HD; Han, S; Kim, KT
- Date Issued
- 1998-04-30
- Publisher
- SPRINGER-VERLAG SINGAPORE PTE LTD
- Abstract
- Mastoparan, a peptide toxin from wasp venome, mimics receptors by stimulating the GTPase activity of guanine nucleotide binding proteins and the G-protein-coupled phospholipase C (PLC), By using Mas-7, the active analog of mastoparan, we showed that it makes pores in the plasma membrane. Treatment with Mas-7 but not Mas-17, the inactive analog, produced a concentration-dependent rise in intracellular Ca2+ concentration ([Ca2+](i)) and facilitated the uptake of ethidium bromide (EtBr) (314 Da) to a sustained level during the stimulation. In addition, Mas-7 triggered the influx of lucifer yellow (457 Da), while it did not induce the influx of fura-2 (831 Da) and Evans blue (961 Da). However, the Mas-7-induced permeability was selectively prevented by the addition of La3+, Ni2+, and Co2+, but not Cd2+. This blocking activity was concentration-dependent. While the stimulatory effect of Mas-7 on PLC activity was dependent on extracellular Ca2+, the pore forming activity of Mas-7 was not effected by removal of extracellular Ca2+ These results, therefore, suggest that the mastoparan's action in pore formation is independent from its action in PLC stimulation and is negatively effected by inorganic cations.
- Keywords
- human neuroblastoma; lanthanum; mastoparan; phospholipase C; pore formation; SK-N-BE(2)C; GTP-BINDING PROTEINS; WASP VENOM; C ACTIVATION; MASTOPARAN; MEMBRANE; RECEPTOR; EXOCYTOSIS; MECHANISMS; PEPTIDES; KINASE
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/20797
- ISSN
- 1016-8478
- Article Type
- Article
- Citation
- MOLECULES AND CELLS, vol. 8, no. 2, page. 162 - 168, 1998-04-30
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