Expression, purification and crystallization of recombinant human TRAIL
SCIE
SCOPUS
- Title
- Expression, purification and crystallization of recombinant human TRAIL
- Authors
- Cha, SS; Shin, HC; Choi, KY; Oh, BH
- Date Issued
- 1999-05
- Publisher
- MUNKSGAARD INT PUBL LTD
- Abstract
- TRAIL (also known as Apo-2L) belongs to the tumour necrosis factor (TNF) cytokine family and induces rapid apoptosis in a wide variety of tumour cell lines upon binding to the death-signalling receptors on the cell membrane. Normal cells are resistant to TRAIL, owing to the expression of decoy receptors which lack functional death domains and antagonize TRAIL-induced apoptosis. Soluble and functional human TRAIL, expressed in Escherichia coli and refolded into a functional form, has been crystallized. The crystals belong to space group P6(3) with unit-cell dimensions a = b = 65.61, c = 131.70 Angstrom. The asymmetric unit contains two molecules of TRAIL, with a crystal volume per protein mass (V-m) of 2.41 Angstrom(3) Da(-1) and a solvent content of about 42% by volume. A native and a platinum-derivative data set to 2.8 and 3.5 Angstrom resolution, respectively, were obtained from frozen crystals. Structure determination by a combined molecular replacement and isomorphous replacement method is in progress.
- Keywords
- TUMOR-NECROSIS-FACTOR; TNF RECEPTOR SUPERFAMILY; INDUCED CELL-DEATH; FACTOR-ALPHA; CRYSTAL-STRUCTURE; MUTATIONAL ANALYSIS; INDUCED APOPTOSIS; LIGAND; FAMILY; DOMAIN
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/20407
- DOI
- 10.1107/S090744499900164X
- ISSN
- 0907-4449
- Article Type
- Article
- Citation
- ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY, vol. 55, page. 1101 - 1104, 1999-05
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